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Updated: Aug 2, 2025

High-throughput Purification of Affinity-tagged Recombinant Proteins
Published on: August 26, 2012
Mediator structure and function in transcription initiation
Srinivasan Rengachari1, Sandra Schilbach1, Patrick Cramer1
1Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Am Fassberg 11, D-37077 Göttingen, Germany.
Abstract:
Recent advances in cryo-electron microscopy have led to multiple structures of Mediator in complex with the RNA polymerase II (Pol II) transcription initiation machinery. As a result we now hold in hands near-complete structures of both yeast and human Mediator complexes and have a better understanding of their interactions with the Pol II pre-initiation complex (PIC). Herein, we provide a summary of recent achievements and discuss their implications for future studies of Mediator and its role in gene regulation.
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