Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA modified by platinum derivatives cannot adopt the A-form.

Z Balcarová, V Brabec

    Biochimica Et Biophysica Acta
    |May 27, 1986
    PubMed
    Summary

    Platinum compounds, including cis-diamminedichloroplatinum(II), alter DNA conformation. These modifications inhibit the B to A transition in DNA, suggesting reduced flexibility upon platinum complex binding.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Triplex metallohelices have enantiomer-dependent mechanisms of action in colon cancer cells.

    Dalton transactions (Cambridge, England : 2003)·2023
    Same author

    Oxidation of cis-Diamminediacetato Pt<sup>II</sup> with Hydrogen Peroxide Can Give Rise to Two Isomeric Pt<sup>IV</sup> Products.

    Chemistry (Weinheim an der Bergstrasse, Germany)·2020
    Same author

    Translesion DNA synthesis across double-base lesions derived from cross-links of an antitumor trinuclear platinum compound: primer extension, conformational and thermodynamic studies.

    Metallomics : integrated biometal science·2017
    Same author

    Assessment of DNA-binding affinity of cholinesterase reactivators and electrophoretic determination of their effect on topoisomerase I and II activity.

    Molecular bioSystems·2016
    Same author

    Comment on "Delivering a photosensitive transplatin prodrug to overcome cisplatin drug resistance" by H. Song, W. Li, R. Qi, L. Yan, X. Jing, M. Zheng and H. Xiao, Chem. Commun., 2015, 51, 11493.

    Chemical communications (Cambridge, England)·2016
    Same author

    Influence of the binding of reduced NAMI-A to human serum albumin on the pharmacokinetics and biological activity.

    Dalton transactions (Cambridge, England : 2003)·2014

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Biophysical Chemistry

    Background:

    • DNA exists in various conformations, including the B-form and A-form.
    • Ethanol can induce a transition from B-DNA to A-DNA.
    • Platinum-based compounds are used in chemotherapy and can interact with DNA.

    Purpose of the Study:

    • To investigate the effect of specific platinum compounds on DNA conformation.
    • To determine if platinum modification affects the ethanol-induced B to A DNA transition.

    Main Methods:

    • Chicken erythrocyte DNA fragments were treated with three platinum compounds: cis-diamminedichloroplatinum(II), its trans-isomer, and chlorodiethylenetriaminoplatinum(II) chloride.
    • Circular dichroism spectroscopy was used to analyze the conformational changes of modified DNA in ethanolic solutions.

    Main Results:

    • Unmodified DNA adopted the A-form in 81% ethanol.
    • All three platinum compounds significantly inhibited the B to A DNA transition induced by high ethanol concentrations.
    • The inhibitory effect of the platinum compounds on the B to A transition was comparable.

    Conclusions:

    • Binding of platinum complexes to B-DNA restricts its conformational freedom.
    • This restriction prevents DNA from adopting the A-conformation in the presence of ethanol.
    • Platinum-DNA interactions have significant implications for DNA structure and function.

    Related Experiment Videos