Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vitamin E inhibits platelet phospholipase A2.

C E Douglas, A C Chan, P C Choy

    Biochimica Et Biophysica Acta
    |May 21, 1986
    PubMed
    Summary

    Vitamin E significantly inhibits platelet phospholipase A2 activity, reducing arachidonic acid release. This effect is dose-dependent and highlights vitamin E

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A distinctive epigenetic ageing profile in human granulosa cells.

    Human reproduction (Oxford, England)·2020
    Same author

    Lister and His Contemporaries in Edinburgh.

    Edinburgh medical journal·2018
    Same author

    Some Observations on Seventy Years of Country Midwifery Practice.

    Transactions. Edinburgh Obstetrical Society·2018
    Same author

    Response to Wang et al. Re: How to choose and compare between radiofrequency ablation and transarterial chemoembolization for recurrent hepatocellular carcinoma?

    HPB : the official journal of the International Hepato Pancreato Biliary Association·2017
    Same author

    The 20 faces of the fourth component of complement.

    Immunology today·2014
    Same author

    Natural killer T cell defects in multiple myeloma and the impact of lenalidomide therapy.

    Clinical and experimental immunology·2013

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Nutrition Science

    Background:

    • Phospholipase A2 (PLA2) releases arachidonic acid for eicosanoid synthesis.
    • Platelet activation involves PLA2-mediated lipid metabolism.
    • Vitamin E is a key antioxidant with potential roles in cellular signaling.

    Purpose of the Study:

    • To investigate the inhibitory effect of vitamin E on platelet phospholipase A2.
    • To elucidate the mechanism underlying vitamin E's inhibition of PLA2.
    • To explore the structural requirements of vitamin E for PLA2 inhibition.

    Main Methods:

    • Dietary administration of vitamin E to rats.
    • Assay of platelet phospholipase A2 activity.
    • Partial purification of rat platelet PLA2 using gel filtration chromatography.
    • In vitro testing of various tocopherol forms on enzyme activity.

    Main Results:

    • Dietary vitamin E (100-1000 ppm) reduced rat platelet PLA2 activity.
    • Exogenous vitamin E further suppressed PLA2 activity in platelet suspensions.
    • Partially purified PLA2 required Ca2+ and was inhibited by tocopherols.
    • Tocol showed greater inhibition than alpha-tocopherols; acetate forms were ineffective.

    Conclusions:

    • Vitamin E inhibits platelet phospholipase A2 in a dose-dependent manner.
    • The hydroxyl group of the chromanol ring is crucial for vitamin E's inhibitory action.
    • Vitamin E plays a significant role in regulating arachidonic acid release and metabolism in platelets.

    Related Experiment Videos