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Related Experiment Videos

[Plasma enzymes inactivating alkaloids].

N R Elaev, L D Evdokimov, A V Ryzhakov

    Biokhimiia (Moscow, Russia)
    |April 1, 1986
    PubMed
    Summary

    Rat blood plasma contains two hemoprotein enzymes that inactivate alkaloids by converting them into N-oxides. These enzymes, differing in polypeptide composition, display cooperative properties.

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    [Blood protein-inactivating atropine].

    Biokhimiia (Moscow, Russia)·1985

    Area of Science:

    • Biochemistry
    • Enzymology

    Context:

    • Alkaloid metabolism in mammals
    • Enzyme kinetics and characterization

    Purpose:

    • To identify and characterize enzymes responsible for alkaloid inactivation in rat plasma.
    • To elucidate the molecular structure and properties of these enzymes.

    Summary:

    • Two distinct hemoprotein enzymes in rat blood plasma were identified.
    • Enzyme 1 comprises three polypeptides (63, 35, 12 kD); Enzyme 2 is a single polypeptide (73 kD).
    • Both enzymes catalyze the transformation of alkaloids into N-oxides and exhibit cooperative kinetics.

    Impact:

    • Provides insight into the detoxification pathways of alkaloids in rats.
    • Characterization of novel hemoproteins with potential implications in drug metabolism research.
    • Understanding enzyme cooperativity in alkaloid biotransformation.