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Updated: Aug 2, 2025

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

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Circular RNA ciRS-7 affects the propagation of Cryptosporidium parvum in HCT-8 cells via regulating miR-135a-5p/stat1

Yan-Ling Yin1, Xin Yang2, Shuang Huang2

  • 1Key Laboratory of Ruminant Disease Prevention and Control (West), College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China; Chongqing Three Gorges Vocational College, Chongqing 404155, China.

Acta Tropica
|April 20, 2023
PubMed

Insights

Host circular RNA ciRS-7 sponges miR-135a-5p, increasing STAT1 levels and promoting Cryptosporidium parvum infection in intestinal cells. This reveals new strategies for controlling cryptosporidiosis.

Area of Science:

  • Molecular Biology
  • Parasitology
  • Host-Pathogen Interactions

Background:

  • Cryptosporidium parvum causes widespread diarrheal disease (cryptosporidiosis) by infecting intestinal epithelial cells.
  • Understanding host-pathogen interactions is crucial for developing control strategies against C. parvum.
  • Previous research identified up-regulated circRNA ciRS-7 promoting C. parvum in vitro.

Purpose of the Study:

  • To elucidate the regulatory role of ciRS-7 and its interaction with microRNAs in C. parvum infection.
  • To investigate the molecular mechanisms by which ciRS-7 influences host cells during C. parvum infection.

Main Methods:

  • Identified miR-135a-5p as a target of ciRS-7.
  • Assessed changes in miR-135a-5p and STAT1 expression (mRNA and protein) post-C. parvum infection.
  • Utilized dual luciferase reporter assays to confirm interactions between ciRS-7, miR-135a-5p, and STAT1.

Main Results:

  • C. parvum infection down-regulated miR-135a-5p and up-regulated STAT1.
  • ciRS-7 directly interacts with miR-135a-5p, and miR-135a-5p targets STAT1.
  • ciRS-7 sponges miR-135a-5p, leading to increased STAT1 and phosphorylated STAT1 (p-STAT1), thereby promoting C. parvum propagation.

Conclusions:

  • Host circRNA ciRS-7 regulates C. parvum infection by sponging miR-135a-5p and up-regulating the STAT1 pathway.
  • This study reveals a novel regulatory mechanism involving host circRNAs in Cryptosporidium infection.
  • Findings offer new insights for developing therapeutic strategies against cryptosporidiosis.
Keywords:
Cryptosporidium parvumPropagationSTAT1ciRS-7miR-135a-5pp-STAT1

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