Sensitive fluorescence assay for the detection of glyphosate with NACCu²⁺ complex

Glyphosate is widely used as an herbicide in weed control. However, the excessive use and residue of glyphosate adversely affect the environment. Thus, a rapid and highly sensitive system must be developed for glyphosate detection. Herein, a novel turn-on fluorescent probe was designed and synthesized for glyphosate, that is N-butyl-1,8-naphthalimide-4-hydrazino-6-isopropyl-chromone (NAC). The fluorescence of NAC was quenched by the addition of Cu²⁺ to form NACCu²⁺ complex in dimethyl sulfoxide/2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (DMSO/HEPES, 9/1, v/v, pH = 7.0). Upon the addition of glyphosate, the fluorescence of NACCu²⁺ was recovered through chelation between Cu²⁺ and glyphosate. The NACCu²⁺ complex exhibited the desired linearity of glyphosate concentration under optimum conditions in the range of 0-40 μM with a low detection limit of 36 nM. Based on competitive coordination, NACCu²⁺ exhibited good sensitivity and selectivity for glyphosate. Moreover, NAC was successfully utilized to detect glyphosate in tap water, local water from Songhua River, soil, maize, and soybean with convenient operations, indicating a promising application in pesticide residue detection.