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Updated: Aug 2, 2025

Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
15:43

Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes

Published on: January 7, 2013

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Kinase perturbations redirect mitochondrial function in cancer

Omar Torres-Quesada1,2, Sophie Strich1, Eduard Stefan1,2

  • 1Tyrolean Cancer Research Institute, Innrain 66, 6020 Innsbruck, Austria.

Bioenergetics Communications
|April 21, 2023
PubMed

Insights

Protein kinases regulate cell processes, and their dysfunction causes cancer. Kinase inhibitors can have off-target effects on mitochondria, impacting cancer drug specificity. Mitochondrial bioenergetics may predict these effects.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Protein kinases are crucial in cell signaling, controlling proliferation, cell cycle, and metabolism.
  • Dysfunctional kinases are implicated in diseases like cancer, leading to kinase inhibitor development.
  • Kinase inhibitors' specificity is vital, but off-target effects can impair drug efficacy.

Purpose of the Study:

  • To review major kinases affecting mitochondrial signaling.
  • To discuss strategies for targeting kinase activities in cancer and mitochondrial function.
  • To explore the potential of mitochondrial bioenergetics in predicting drug effects.

Main Methods:

  • Literature review of kinase functions and mitochondrial signaling.
  • Analysis of pharmacological strategies targeting kinases and mitochondria.
  • Discussion of cell-based assays for monitoring mitochondrial bioenergetics.

Main Results:

  • Selected major kinases significantly impact mitochondrial signaling pathways.
  • Kinase inhibition can converge on mitochondria, affecting cellular energy.
  • Pharmacological strategies aim to redirect mitochondrial function in cancer therapy.

Conclusions:

  • Understanding kinase-mitochondria interactions is key for cancer drug development.
  • Cell-based mitochondrial bioenergetic recordings can predict kinase inhibitor off-target effects.
  • Targeting mitochondrial function offers a strategy to enhance cancer therapy specificity.
Keywords:
cancerdrug off-target effectskinase inhibitorskinasesmitochondriasignaling

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