Tahibacter harae sp. nov., isolated from pig farm soil in Guangdong, PR China

In the process of exploring the microbial diversity of pig farms, a Gram-stain-negative, aerobic, rod-shaped, non-spore-forming, non-motile bacterial strain, designated P2K^T, was isolated from soil sample collected at a pig farm, Guangdong Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P2K^T belonged to the genus Tahibacter, with the highest sequence similarity to Tahibacter aquaticus PYM5-11^T (98.6%) and Tahibacter caeni BUT-6^T (98.3 %). The genome size of strain P2K^T was 6.0 Mb with a DNA G+C content of 68.3 mol%. Average nucleotide identity values between strain P2K^T and the type strains of the genus Tahibacter were 81.1-81.6 %. The digital DNA-DNA hybridization values between P2K^T and these relative species were 24.5-25.6%. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, an unknown aminolipids, two unknown lipids and three unknown phospholipids. The major respiratory quinone of strain P2K^T was ubiquinone Q-8, and the main fatty acids (>10.0 % of total fatty acids) of strain P2K^T were iso-C_15:0, iso-C_16:0 and summed feature 9 (C_16:0 10-methyl and/or iso-C_17:1 ω9c). Based on phenotypic and genotypic data, strain P2K^T represents a novel species within the genus Tahibacter, for which the name Tahibacter harae sp. nov. is proposed, with the type strain P2K^T (=GDMCC 1.3107^T=JCM 35231^T).