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A methane-cGMP module positively influences adventitious rooting.

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Methane (CH4) is a natural plant and microbial product.
  • Exogenous methane (CH4) and cyclic guanosine monophosphate (cGMP) individually influence adventitious root (AR) formation.
  • The interaction between CH4 and cGMP in AR development was previously unclear.

Purpose of the Study:

  • To investigate the interaction between methane (CH4) and cyclic guanosine monophosphate (cGMP) in adventitious root (AR) formation.
  • To elucidate the molecular mechanisms by which CH4 influences AR development via cGMP.

Main Methods:

  • Treatment of plants with exogenous methane (CH4) and cGMP derivatives.
  • Inhibition of guanosine cyclase (GC) activity to assess endogenous cGMP roles.
  • Genetic manipulation using transgenic Arabidopsis overexpressing methyl-coenzyme M reductase (MtMCR).
  • Quantitative PCR (qPCR) analysis of gene expression related to cell cycle and auxin signaling.

Main Results:

  • Exogenous CH4 rapidly increased cGMP synthesis and promoted cucumber AR development.
  • Inhibition of endogenous cGMP impaired CH4-induced AR formation.
  • Transgenic Arabidopsis overexpressing MtMCR showed enhanced AR development, particularly with CH4 or 8-Br-cGMP.
  • CH4-cGMP signaling modulated the expression of cell cycle and auxin signaling genes.

Conclusions:

  • Endogenous cGMP acts downstream of CH4 in controlling adventitious rooting.
  • The CH4-cGMP pathway regulates AR formation by modulating auxin signaling and cell cycle progression.
  • This study reveals a novel molecular mechanism for CH4 in plant development.