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Perspectives on improving crop Rubisco by directed evolution.

Matteo Gionfriddo1, Timothy Rhodes2, Spencer M Whitney2

  • 1Plant Science Division, Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia; Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Martinsried 82152, Germany.

Seminars in Cell & Developmental Biology
|April 21, 2023
PubMed
Summary
This summary is machine-generated.

Improving Rubisco, crucial for photosynthesis, is key to boosting crop yields. Researchers are exploring directed evolution to enhance Rubisco

Keywords:
CO(2)-fixationCalvin cycleChaperonesChaperoninChloroplast transformationDirected evolutionFood securityPhotosynthesisSynthetic biology

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Area of Science:

  • Biochemistry
  • Plant Science
  • Molecular Biology

Background:

  • Rubisco is essential for carbon fixation in photosynthesis but is slow and inefficient.
  • Its inefficiency necessitates high production levels, impacting plant growth.
  • More efficient Rubisco variants exist in algae, but transplantation to plants is challenging.

Purpose of the Study:

  • To review advances in improving Rubisco catalysis through directed evolution.
  • To discuss challenges in enhancing solubility and carboxylation activity of non-plant Rubisco.
  • To explore opportunities for directed evolution of crop Rubiscos and assess potential agricultural benefits.

Main Methods:

  • Review of current literature on Rubisco engineering.
  • Analysis of directed evolution strategies for non-plant Rubisco.
  • Discussion of plant transformation capabilities for evaluating improved Rubisco.

Main Results:

  • Significant progress has been made in improving Rubisco from various lineages via directed evolution.
  • Challenges remain in enhancing solubility and carboxylation efficiency.
  • Algal Rubisco's potential is hindered by chloroplast incompatibilities.

Conclusions:

  • Directed evolution offers a promising avenue for enhancing Rubisco activity.
  • Overcoming protein folding incompatibilities is crucial for utilizing algal Rubisco in plants.
  • Improving Rubisco holds significant potential for increasing agricultural productivity.