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Published on: October 2, 2016
Summary
This review covers chromatographic and homogeneous immunoassay techniques used in clinical labs. It focuses on their principles for quantitatively measuring therapeutic drugs in patient serum.
Area of Science:
- Clinical Chemistry
- Analytical Chemistry
- Biomedical Science
Background:
- Chromatographic and homogeneous immunoassay methods are standard in clinical laboratories.
- Accurate quantification of therapeutic drugs in serum is crucial for patient management.
Purpose of the Study:
- To review the fundamental principles of chromatographic and homogeneous immunoassay methods.
- To highlight their specific applications in the quantitative analysis of therapeutic drugs within serum samples.
Main Methods:
- Review of established chromatographic techniques (e.g., HPLC, GC).
- Review of homogeneous immunoassay principles (e.g., EMIT, FPIA).
- Focus on principles relevant to serum drug level determination.
Main Results:
- Detailed explanation of the separation and detection mechanisms in chromatography.
- Explanation of antigen-antibody binding kinetics and signal generation in immunoassays.
- Comparison of method suitability for various therapeutic drug classes.
Conclusions:
- Both chromatographic and homogeneous immunoassays are vital tools for therapeutic drug monitoring.
- Understanding their core principles facilitates appropriate method selection for accurate serum drug quantification.
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