MitoQ ameliorates PM_2.5-induced pulmonary fibrosis through regulating the mitochondria DNA homeostasis

Pulmonary fibrosis is a severe pulmonary disease, and may related to PM_2.5 exposure. Our study aims to explore the pathogenesis of PM_2.5-induced pulmonary fibrosis, and MitoQ protective effect in this process. Our results find that inflammatory cells aggregation and pulmonary fibrosis in mice lung after PM_2.5 exposure. Moreover, Collagen I/III overproduction, EMT and TGF-β1/Smad2 pathway activation in mice lung and BEAS-2B after PM_2.5 exposure. Fortunately, these changes were partially ameliorated after MitoQ treatment. Meanwhile, severe oxidative stress, mitochondrial homeostasis imbalance, overproduction of 8-oxoG (7,8-dihydro-8-oxoguanine), as well as the inhibition of SIRT3/OGG1 pathway have founded in mice lung or BEAS-2B after PM_2.5 exposure, which were alleviated by MitoQ treatment. Collectively, our study found that oxidative stress, especially mitochondrial oxidative stress participates in the PM_2.5-induced pulmonary fibrosis, and MitoQ intervention had a protective effect on this progress. Moreover, mitochondrial DNA homeostasis might participate in the pulmonary fibrosis caused by PM_2.5 exposure. Our study provides a novel pathogenesis of PM_2.5-caused pulmonary fibrosis and a possible targeted therapy for the pulmonary diseases triggered by PM_2.5.