Related Experiment Videos
Abstract:
In this liquid-chromatographic assay for hemoglobin A2 (Hb A2) in human blood, the blood samples are hemolyzed by dilution and the hemolysates are chromatographed on a weak cation-exchanger with a solvent gradient of a buffer with two different ionic strengths. The absorbance of the effluent is monitored at 405 nm. The retention time of Hb A2 is less than 10 min. The mean within-assay CV was 1.6%, the between-assay CV 4.9%. I describe the effects of various column dimensions and sample preparation methods on the separation of Hb A2. The normal reference interval (for 54 healthy adults) was 1.3 to 3.0%. A comparison between results by a minicolumn method and by the present method is performed. Rapid and reproducible, this method is suitable for use in routine determination of Hb A2.