Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[The role of endoplasmic reticulum stress in insulin resistance of type 2 diabetes].

Sheng li ke xue jin zhan [Progress in physiology]·2012

Same author

EVI1 acts as an inducible negative-feedback regulator of NF-κB by inhibiting p65 acetylation.

Journal of immunology (Baltimore, Md. : 1950)·2012

Same author

Cu(II)-catalyzed asymmetric hydrosilylation of diaryl- and aryl heteroaryl ketones: application in the enantioselective synthesis of orphenadrine and neobenodine.

Chemistry (Weinheim an der Bergstrasse, Germany)·2012

Same author

T-bet acts as a powerful adjuvant in Ag85B DNA‑based vaccination against tuberculosis.

Molecular medicine reports·2012

Same author

Aspirin-triggered lipoxin A₄ attenuates lipopolysaccharide-induced intracellular ROS in BV2 microglia cells by inhibiting the function of NADPH oxidase.

Neurochemical research·2012

Same author

Preparation and time-gated luminescence bioimaging applications of long wavelength-excited silica-encapsulated europium nanoparticles.

Nanoscale·2012

Search research articles

Related Experiment Video

Updated: Aug 1, 2025

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

Advances in ultrahigh-throughput screening technologies for protein evolution.

Zhanzhi Liu¹, Sheng Chen¹, Jing Wu¹

¹State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu Province, China; School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu Province, China; International Joint Laboratory on Food Safety, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu Province, China.

Trends in Biotechnology

|April 23, 2023

Summary

Directed evolution enhances protein properties through gene mutation and screening. This review focuses on ultrahigh-throughput screening technologies, a key factor limiting directed evolution progress.

Keywords:

directed evolution genotype–phenotype coupling ultrahigh-throughput screening

More Related Videos

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

Related Experiment Videos

Last Updated: Aug 1, 2025

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

Area of Science:

Biotechnology and molecular biology, focusing on protein engineering.

Background:

Directed evolution mimics natural selection to improve protein function.
Key steps include creating diverse gene libraries and efficient screening.
Protein property variations necessitate specialized screening methods, which are a bottleneck.

Purpose of the Study:

To review ultrahigh-throughput screening principles based on protein properties.
To comprehensively introduce recent advancements and future directions in screening technologies for directed evolution.

Main Methods:

Organizing screening principles from a protein property perspective.
Reviewing and synthesizing the latest progress in ultrahigh-throughput screening technologies.
Analyzing future trends in directed evolution screening.

Main Results:

Established a framework for understanding screening based on protein characteristics.
Detailed the current state-of-the-art in ultrahigh-throughput screening for directed evolution.
Identified key areas for future technological development.

Conclusions:

Ultrahigh-throughput screening is crucial for accelerating directed evolution.
Advances in screening technologies are vital for overcoming limitations in protein engineering.
Future research should focus on developing more efficient and versatile screening platforms.