Related Experiment Video
Updated: Aug 1, 2025
Comprehensive Autopsy Program for Individuals with Multiple Sclerosis
Published on: July 19, 2019
Abnormal expression of MAPK14-related lncRNAs in the peripheral blood of patients with multiple sclerosis
Soudeh Ghafouri-Fard1, Mahdi Gholipour2, Solat Eslami3,4
1Men's Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Introduction:
Contribution of MAPK14 in the pathogenesis of multiple sclerosis (MS) has been proposed by several studies. Long non-coding RNA (lncRNA) have been suggested to be functionally linked with Mitogen-activated protein kinase 14 (MAPK14).
Methods:
Expression levels of MAPK14 and its associated lncRNAs were measured in the circulation of MS patients compared with control subjects.
Results:
Expression levels of NORAD and RAD51-AS1 were higher in total patients compared with controls (Expression ratio (95% CI) = 1.4 (1.04-1.89), P value = 0.015 and Expression ratio (95% CI) = 1.91 (1.43-2.6), P value = 0.0001, respectively). Conversely, ZNRD1ASP was under-expressed in cases compared with controls (Expression ratio (95% CI) = 0.61 (0.41-0.8), P value = 0.0005). In spite of the observed abnormal expression levels of these lncRNAs in the circulation of MS patients, their expressions were not correlated with Expanded Disability Status Scale (EDSS) score, disease duration or age at disease onset.
Conclusion:
To sum up, the current investigation shows dysregulation of MAPK14-related lncRNAs in MS patients.
Insights
Multiple sclerosis (MS) pathogenesis involves MAPK14. This study found altered expression of MAPK14-related long non-coding RNAs (lncRNAs) in MS patients, suggesting their role in the disease.
Area of Science:
- Neuroimmunology
- Molecular Biology
- Genetics
Background:
- Mitogen-activated protein kinase 14 (MAPK14) is implicated in multiple sclerosis (MS) pathogenesis.
- Long non-coding RNAs (lncRNAs) are increasingly recognized for their functional links to kinase pathways, including MAPK14.
Purpose of the Study:
- To investigate the expression levels of MAPK14-associated lncRNAs in the circulation of MS patients.
- To determine if these lncRNAs correlate with clinical parameters of MS.
Main Methods:
- Quantitative analysis of MAPK14 and specific lncRNAs (NORAD, RAD51-AS1, ZNRD1ASP) in blood samples from MS patients and healthy controls.
- Statistical analysis to compare expression levels and assess correlations with disease severity (EDSS), duration, and age at onset.
Main Results:
- NORAD and RAD51-AS1 showed significantly higher expression in MS patients compared to controls (p=0.015 and p=0.0001, respectively).
- ZNRD1ASP was significantly downregulated in MS patients (p=0.0005).
- No significant correlation was found between the expression of these lncRNAs and EDSS score, disease duration, or age at disease onset.
Conclusions:
- The study demonstrates dysregulation of specific MAPK14-related lncRNAs in the circulation of individuals with multiple sclerosis.
- These findings highlight potential novel biomarkers or therapeutic targets in MS, although their direct correlation with disease progression requires further investigation.
More Related Videos
12:49Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation
Published on: March 21, 2020
07:24Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos
Published on: December 13, 2018
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
01:37Neural Regulation
01:07MAPK Signaling Cascades