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Related Experiment Video

Updated: Aug 1, 2025

Dissection of Larval Zebrafish Gonadal Tissue

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Published on: April 26, 2017

Comparative gonad transcriptome analysis in cobia (Rachycentron canadum).

Xueyan Shen¹, José M Yáñez², Giana Bastos Gomes³

¹Tropical Futures Institute, James Cook University Singapore, Singapore, Singapore.

Frontiers in Genetics

|April 24, 2023

Summary

This study identified key genes and pathways involved in cobia sexual development. Findings provide a molecular basis for understanding cobia sex determination and developing monosex female aquaculture.

Keywords:

Rachycentron canadum cobia gonad transcriptome reproductive related pathways sex-biased genes sexual size dimorphism

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Area of Science:

Marine Biology
Aquaculture Genetics
Comparative Genomics

Background:

Cobia (Rachycentron canadum) exhibits rapid growth and sexual size dimorphism favoring females, suggesting aquaculture benefits from monosex female populations.
Understanding the genetic basis of sex determination and gonadal development in cobia is crucial for selective breeding.
Current knowledge regarding the genetic factors influencing cobia sexual development is limited.

Purpose of the Study:

To identify sex-biased differentially expressed genes (DEGs) in cobia testes and ovaries using transcriptome sequencing.
To elucidate the molecular mechanisms underlying sex determination and gonadal differentiation in cobia.
To provide a foundation for developing strategies for monosex female cobia production in aquaculture.

Main Methods:

Comparative gonad transcriptome sequencing of cobia (Rachycentron canadum).
Identification and annotation of sex-biased differentially expressed genes (DEGs).
Validation of candidate gene expression using quantitative PCR (qPCR).

Main Results:

Transcriptome analysis revealed 7,120 and 4,628 DEGs up-regulated in testes and ovaries, respectively.
Identified 76 candidate genes involved in sex determination and differentiation, including known testis-biased (e.g., dmrt1, amh, sox9) and ovary-biased (e.g., foxl2, sox3, cyp19a) genes.
Discovered eleven enriched pathways related to sex determination and differentiation, such as Wnt signaling and oocyte meiosis.

Conclusions:

This is the first comparative gonad transcriptome study in cobia, offering novel insights into its sexual development.
The identified sex-associated DEGs and pathways provide a molecular basis for investigating cobia sex determination.
Findings support future research into gonadal development and the controlled breeding of monosex female cobia for aquaculture.