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Related Experiment Video

Updated: Aug 1, 2025

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

Hydrogel particles-on-chip (HyPoC): a fluorescence micro-sensor array for IgG immunoassay.

Alessandra De Masi^1,2, Pasqualina Liana Scognamiglio¹, Edmondo Battista^1,3

¹Center for Advanced Biomaterials for Healthcare@CRIB, Istituto Italiano di Tecnologia (IIT), Largo Barsanti e Matteucci 53, 80125 Naples, Italy. pasqualina.scognamiglio@unibas.it.

|April 24, 2023

Summary

This summary is machine-generated.

Novel hydrogel microparticles enable rapid, sensitive detection of human immunoglobulin G in biological fluids. This lab-on-chip platform offers tuneable specificity and sensitivity for point-of-care diagnostics.

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Area of Science:

Biomedical Engineering
Analytical Chemistry
Materials Science

Background:

Microparticles offer high sensitivity and specificity for biomolecule detection.
Hydrogel microparticles, especially PEG-based, are promising for biosensors due to functionalization capabilities.
Limitations in particle manipulation in microfluidics hinder point-of-care applications.

Purpose of the Study:

To develop an on-chip sandwich immunoassay for detecting human immunoglobulin G (hIgG).
To engineer cleavable PEG-based microparticles for enhanced detection.
To create a microfluidic device for precise particle manipulation and rapid diagnostics.

Main Methods:

Developed cleavable PEG-based microparticles functionalized with monoclonal antibodies.
Integrated functionalized particles into a microfluidic device (HyPoC) for controlled positioning.
Performed on-chip sandwich immunoassay for hIgG detection in serum and urine.

Main Results:

Achieved tuneable specificity and sensitivity down to 3 pM by adjusting particle numbers.
Demonstrated a limit of detection of 0.07 nM with a dynamic range of 0.07-1 nM.
Reduced incubation time from 180 minutes to 1 minute and washing volumes to 90 μL.

Conclusions:

The developed HyPoC platform enables versatile, fast, and easy point-of-care testing for immunoassays.
Precisely controlled hydrogel microparticles in microfluidics significantly improve diagnostic efficiency.
This approach overcomes limitations in particle manipulation for advanced diagnostic devices.