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Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

Published on: December 15, 2017

SNAP-Tag-Targeted MRI-Fluorescent Multimodal Probes.

Ebaston Thankarajan^1,2, Shimrit Oz¹, Abed Saady^1,2,3

¹Department of Neuroscience, Rappaport Faculty of Medicine, Technion - Israel Institute of Technology, Haifa, 3525422, Israel.

Chembiochem : a European Journal of Chemical Biology

|April 24, 2023

Summary

Researchers developed a novel multimodal imaging probe for enhanced cell detection. This probe combines magnetic resonance imaging (MRI) contrast with bright fluorescence for specific cell labeling in microscopy and MRI applications.

Keywords:

MRI SNAP-tag contrast agents fluorescent probes imaging agents

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Last Updated: Aug 1, 2025

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

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Published on: December 15, 2017

Hybrid µCT-FMT imaging and image analysis

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In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles

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Published on: May 2, 2014

Area of Science:

Multimodal imaging probe development
Cellular and molecular imaging
Biomedical engineering

Background:

Magnetic resonance imaging (MRI) offers powerful visualization but lacks signal strength and target specificity.
Existing imaging techniques often require separate probes for different modalities, complicating workflows.
Need for integrated probes that enhance both MRI contrast and fluorescence detection.

Purpose of the Study:

To synthesize a trifunctional imaging probe for simultaneous MRI and fluorescence microscopy.
To achieve specific and efficient labeling of genetically defined cells.
To create a versatile platform for multimodal imaging probe production.

Main Methods:

Synthesis of a trifunctional probe incorporating a SNAP-tag substrate, cyanine dyes, and a gadolinium(III) chelate.
Utilizing SNAP-tag for irreversible and specific cell membrane labeling.
Evaluating probe performance in fluorescence microscopy and MRI.

Main Results:

The synthesized probe demonstrated specific and efficient labeling of SNAP-tag expressing cells.
Probes provided bright fluorescence signals for microscopy.
Enhanced MRI contrast was achieved, indicating successful signal amplification.
The synthetic strategy proved versatile for creating multimodal probes.

Conclusions:

The developed trifunctional probe effectively enhances cell detection through combined MRI and fluorescence imaging.
The synthetic approach offers a flexible platform for creating advanced multimodal imaging agents.
This technology holds promise for improved cellular imaging in research and clinical settings.