Related Experiment Video
Updated: Aug 1, 2025
Step By Step: Microsurgical training method combining two nonliving animal models
Published on: May 9, 2015
Towards a standardized initial training program in experimental microsurgery for pediatric surgeons
M Velayos1, K Estefanía-Fernández1, A J Fernández Bautista1
1Pediatric Surgery Department. Hospital Universitario La Paz. Madrid (Spain). Instituto de Investigación. Hospital Universitario La Paz, IdiPAZ. Madrid (Spain).
Objective:
To describe a basic training program in microsurgery and to analyze the learning curve through the process, including improvement in operating times and functional outcome.
Materials And Methods:
Our learning program included basic, transitional, and experimental models. The experimental model included tail vein cannulation, intestinal resection and anastomosis, dissection, division and anastomosis of the cava and aorta. Wistar rats (66.7% male; 406.9 ± 38.9 grams) were used. The program adhered to the 3R principle and obtained animal welfare committee approval.
Results:
Mean tail vein cannulation time was 2.4 ± 1.2 minutes. Mean intestinal resection and jejunocolic anastomosis time was 14.8 ± 2.7 minutes and 10.4 ± 3 minutes, respectively. All anastomoses were functionally valid. Mean vessel dissection time was 22.9 ± 7.7 minutes, aortic artery anastomosis was 17.2 ± 7.1 minutes, and vena cava anastomosis was 25.9 ± 7.3 minutes. 66.7% of vena cava anastomoses were functionally valid vs. 88.9% for the aorta. The time required for all procedures decreased after the third attempt, except for vena cava anastomoses, which remained similar in all 9 procedures.
Conclusions:
Our model demonstrated that the procedures were suitable for trainer progression in terms of surgical time and functional outcome. Microsurgical training would benefit from standardized programs to optimize results.
