Carbon dot and silver nanoparticle-based fluorescent probe for the determination of sulfite and bisulfite via...

A new sensitive fluorescent probe (CDs-AgNP/H₂O₂) for detecting sulfite and bisulfite (SO₃^2- and HSO₃^-) based on the inner-filter effect (IFE) between silver nanoparticles (AgNPs) and carbon dots (CDs) was developed. Because of the spectral overlap between the absorption of AgNPs and the excitation of CDs, the fluorescence of CDs can be quenched by AgNPs owing to the IFE. H₂O₂ weakens the IFE and restores the fluorescence due to the oxidation of AgNPs by H₂O₂. However, the existence of SO₃^2-/HSO₃^- can quench the fluorescence again as a result of redox reaction between SO₃^2-/HSO₃^- and H₂O₂. The results showed a broad linear range of 20-200 μM with a low limit of detection (3.02 μM) toward SO₃^2-/HSO₃^-. The combination of IFE and redox reaction led to improvement of the sensitivity and selectivity. The probe was implemented to measure SO₃^2-/HSO₃^- in various agricultural products and foods with acceptable results (80.6 to 118.9% recovery).