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Related Experiment Video

Updated: Aug 1, 2025

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
08:57

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Published on: July 17, 2021

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Recombinant Protein Production Using the Baculovirus Expression Vector System (BEVS).

Heather McDuffie Sullivan1, Ewa Krupinska1, Anna Andersson Rasmussen1

  • 1Lund Protein Production Platform (LP3) & Protein Production Sweden (PPS), Department of Biology, Lund University, Lund, Sweden.

Methods in Molecular Biology (Clifton, N.J.)
|April 24, 2023
PubMed
Summary
This summary is machine-generated.

We present reproducible methods for frequently producing diverse recombinant proteins using the baculovirus expression vector system (BEVS) for research applications. Our platform efficiently generates small to medium quantities of proteins, supporting various scientific investigations.

Keywords:
Baculovirus-infected insect cells (BIIC)Cell cultureG protein-coupled receptorsInsect cellsProtein Production Sweden (PPS)Protein complexesProtein kinasesStructural biology

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Protein Expression

Background:

  • The baculovirus expression vector system (BEVS) is a widely adopted eukaryotic system for producing recombinant proteins.
  • Research applications often necessitate small to medium quantities of diverse proteins, ranging from micrograms to milligrams.

Purpose of the Study:

  • To detail standard operating procedures and platform setup for frequent and repeatable recombinant protein production.
  • To enable the generation of multiple protein types for research use.

Main Methods:

  • Implementation of established baculovirus expression vector system protocols.
  • Standardized platform setup for consistent protein yield.
  • Methodologies optimized for frequent and repeated protein generation.

Main Results:

  • Demonstrated ability to frequently produce various recombinant proteins.
  • Successful generation of protein quantities in the range of tens of micrograms to a few hundred milligrams.
  • Established a reliable workflow for diverse protein production.

Conclusions:

  • The presented methods provide a robust and efficient platform for recombinant protein production via BEVS.
  • The system supports the frequent and repeatable generation of diverse proteins for research purposes.
  • This platform facilitates accessibility to a wide range of recombinant proteins for scientific investigation.