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MTSviewer: A database to visualize mitochondrial targeting sequences, cleavage sites, and mutations on protein
Andrew N Bayne1, Jing Dong1, Saeid Amiri2
1Department of Pharmacology & Therapeutics and Centre de Recherche en Biologie Structurale, McGill University, Montréal, Quebec, Canada.
Plos One
|April 24, 2023
Summary
Mitochondrial targeting sequences (MTSs) guide protein import into mitochondria. A new database, MTSviewer, now characterizes the genetic and structural landscape of these crucial sequences, aiding disease research.
Area of Science:
- Mitochondrial Biology
- Molecular Genetics
- Bioinformatics
Background:
- Mitochondrial dysfunction contributes to numerous human diseases.
- Protein import into mitochondria relies on mitochondrial targeting sequences (MTSs).
- Internal MTSs present novel regulatory roles beyond N-terminal sequences.
Purpose of the Study:
- To characterize the global mutational landscape of MTSs.
- To develop a comprehensive database for MTS analysis.
- To integrate genetic, structural, and functional data for mitochondrial proteins.
Main Methods:
- Development of the R/Shiny database MTSviewer.
- Integration of MTS prediction, cleavage site analysis, and genetic variant data.
- Utilized AlphaFold models for structural visualization of human and yeast mitochondrial proteomes.
Main Results:
- MTSviewer provides a unified platform for MTS analysis.
- Profiled MTS-containing proteins across human and yeast proteomes.
- Demonstrated utility through case studies highlighting MTS variations.
Conclusions:
- MTSviewer enhances understanding of MTSs and their role in mitochondrial function.
- The database facilitates research into diseases linked to mitochondrial dysfunction.
- Characterizing MTSs offers new avenues for genetic and structural studies.
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