Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spheroids with Artificial Mineral Organelles: Machine-Learning-Assisted Analysis and Control of Mechanical Properties, Fusion, and Ossification.

ACS applied materials & interfaces·2026
Same author

Correlative ultrastructural mapping of Lewy pathology reveals regional diversity in Parkinson's and dementia with Lewy bodies.

Acta neuropathologica·2026
Same author

Kainic acid model of temporal lobe epilepsy: Nanoscale oscillations of hippocampal mitochondria.

Tissue & cell·2026
Same author

Rapid Optical Nanomotion-Based Antibiotic Susceptibility Testing of Kombucha-Associated Acetic Acid Bacteria and <i>Escherichia coli</i>.

Foods (Basel, Switzerland)·2026
Same author

Nanomotion-Based Drug Sensitivity Prediction in Ovarian and Colon Cancer Cell Lines Using Machine Learning.

ACS pharmacology & translational science·2025
Same author

Rapid assessment of bisphenol A toxicity on fish eggs using optical nanomotion detection.

Environmental toxicology and chemistry·2025

Related Experiment Video

Updated: Aug 1, 2025

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
08:33

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Published on: August 5, 2020

7.0K

Simple optical nanomotion method for single-bacterium viability and antibiotic response testing.

Maria I Villalba1,2, Eugenia Rossetti3, Allan Bonvallat3

  • 1Laboratory of Biological Electron Microscopy, Ecole Polytechnique Fédérale de Lausanne, Lausanne 1015, Switzerland.

Proceedings of the National Academy of Sciences of the United States of America
|April 24, 2023
PubMed
Summary

A new optical microscopy method rapidly detects bacterial antibiotic resistance at the single-cell level. This accessible technique offers a faster, cheaper alternative for antimicrobial susceptibility testing (AST) to combat public health threats.

Keywords:
antibioticbacteriananomotionoptical microscopy

More Related Videos

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
11:52

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro

Published on: April 21, 2023

3.2K
A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
06:18

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials

Published on: March 3, 2023

1.5K

Related Experiment Videos

Last Updated: Aug 1, 2025

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
08:33

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Published on: August 5, 2020

7.0K
Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
11:52

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro

Published on: April 21, 2023

3.2K
A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
06:18

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials

Published on: March 3, 2023

1.5K

Area of Science:

  • Microbiology
  • Biophysics
  • Medical Diagnostics

Background:

  • Antibiotic resistance is a critical global health challenge.
  • Rapid antimicrobial susceptibility testing (AST) is crucial for effective treatment.
  • Current AST methods often lack speed, affordability, or accessibility.

Purpose of the Study:

  • To develop a rapid, single-cell sensitive, and accessible method for AST.
  • To present a novel nanomotion detection technique using optical microscopy.
  • To overcome limitations of existing AST approaches.

Main Methods:

  • Utilizing optical microscopy to monitor bacterial nanomotion.
  • Employing a basic microfluidic analysis chamber and standard optical microscope.
  • No bacterial attachment, stains, or markers are required for analysis.

Main Results:

  • Successfully performed single-cell AST for diverse bacteria (motile, nonmotile, Gram-positive, Gram-negative).
  • Demonstrated a rapid and highly efficient detection of bacterial viability.
  • Validated a technique that is simple to implement and widely accessible.

Conclusions:

  • The developed optical microscopy method offers a game-changing approach to rapid AST.
  • This technique provides a cost-effective and user-friendly solution for identifying antibiotic resistance.
  • The method holds significant potential for improving infectious disease management globally.