New insights into hexavalent chromium exposure in electron donor limited denitrification: bio-electron behavior

The bio-electron behavior (electron production, transmission, and consumption) response to a typical heavy metal, hexavalent chromium, was unraveled in the electron donor limited system (EDLS) and electron donor sufficient system (EDSS). Nicotinamide adenine dinucleotide and adenosine triphosphate production were reduced by 44% and 47%, respectively, due to glucose metabolism inhibition, leading to NO₃^--N declining to 31% in EDLS. The decreased electron carrier contents and denitrifying enzymes activity inhibited electron transmission and consumption in both EDLS and EDSS. Additionally, electron transfer and antioxidant stress abilities were reduced, further hindering the survival of denitrifiers in EDLS. The lack of dominant genera (Comamonas, Thermomonas, and Microbacterium) in EDLS was the primary reason for poor biofilm formation and chromium adaptability. The decreased expression of enzymes related to glucose metabolism caused the imbalance of electron supply, transport, and consumption in EDLS, adversely impacting nitrogen metabolism and inhibiting denitrification performance.