Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Lines01:16

Cell Lines

7.6K
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
7.6K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.2K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.2K
Nondisjunction01:21

Nondisjunction

3.9K
Nondisjunction is the failure of homologous chromosomes or sister chromatids to separate correctly and move to the opposite poles of the cells. This produces daughter cells with abnormal chromosome numbers.  Nondisjunction is common during anaphase I or anaphase II of meiosis.  Mutations in synaptonemal complex proteins that attach homologous chromosomes increase the chances of nondisjunction in anaphase I of meiosis I. In contrast, mutations in topoisomerases and condensins that hold...
3.9K
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

12.3K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
12.3K
Meiosis vs. Mitosis02:57

Meiosis vs. Mitosis

57.3K
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
57.3K
Mutagenicity and Carcinogenicity01:25

Mutagenicity and Carcinogenicity

1.3K
Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Complete mitochondrial genome of the Australian basket clam <i>Corbicula australis</i>, an exotic species found in New Zealand.

Microbiology resource announcements·2026
Same author

Near-complete genome sequence of <i>Mycoplasma meleagridis</i> isolated from a New Zealand turkey.

Microbiology resource announcements·2026
Same author

Lupus nephritis outcomes in Aotearoa New Zealand, a retrospective case series.

Lupus·2026
Same author

Development of a portable avian influenza virus characterisation system: bringing the inside-out.

Scientific reports·2025
Same author

Genome sequence of <i>Babesia gibsoni</i> detected in a domestic dog in New Zealand.

Microbiology resource announcements·2025
Same author

Phylogenetic and Molecular Characterization of a Novel Reassortant High-Pathogenicity Avian Influenza A (H7N6) Virus Detected in New Zealand Poultry.

International journal of molecular sciences·2025
Same journal

Development of a Tumor-Bearing Animal Model to Evaluate Chemotherapy Efficacy and Toxicity.

Cancer reports (Hoboken, N.J.)·2026
Same journal

CCT7 Expression Affects the Prognosis of Lung Adenocarcinoma.

Cancer reports (Hoboken, N.J.)·2026
Same journal

Pembrolizumab-Induced Myositis: Diagnostic and Therapeutic Challenges From Two Case Reports and a Narrative Review.

Cancer reports (Hoboken, N.J.)·2026
Same journal

Case Series: Can Daily Patient-Reported Outcome Measures Be Implemented for Cancer-Related Fatigue?

Cancer reports (Hoboken, N.J.)·2026
Same journal

Impact of Frontline Treatment Strategies on Outcomes in Patients With Acute Myeloid Leukemia, Myelodysplasia-Related.

Cancer reports (Hoboken, N.J.)·2026
Same journal

The Utility of HER2 Overexpression in Prognosis of Gastric Cancer: A Systematic Review and Meta-Analysis Study.

Cancer reports (Hoboken, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Aug 1, 2025

Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes
05:22

Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes

Published on: April 13, 2018

10.5K

Chromosomal instability and its effect on cell lines.

Zichen He1, Andrew Wilson1, Fenella Rich1

  • 1Department of Pathology and Molecular Medicine, University of Otago, Wellington, New Zealand.

Cancer Reports (Hoboken, N.J.)
|April 24, 2023
PubMed
Summary
This summary is machine-generated.

Chromosomal instability (CIN) in cancer cell lines causes genetic heterogeneity, impacting research reproducibility. This review details CIN causes and offers strategies for monitoring and control in cell culture.

Keywords:
cell culturecell lineschromosome instabilitymitotic checkpointtelomere

More Related Videos

Chromosome Preparation From Cultured Cells
07:42

Chromosome Preparation From Cultured Cells

Published on: January 28, 2014

81.5K
Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
07:54

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Published on: August 19, 2014

17.1K

Related Experiment Videos

Last Updated: Aug 1, 2025

Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes
05:22

Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes

Published on: April 13, 2018

10.5K
Chromosome Preparation From Cultured Cells
07:42

Chromosome Preparation From Cultured Cells

Published on: January 28, 2014

81.5K
Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
07:54

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Published on: August 19, 2014

17.1K

Area of Science:

  • Cell Biology
  • Genetics
  • Cancer Research

Background:

  • Cancer cell lines are vital for research but face reproducibility challenges.
  • Chromosomal instability (CIN) is a key issue, leading to genetic heterogeneity.
  • This heterogeneity can compromise the reliability of in vitro cancer models.

Purpose of the Study:

  • To review the underlying causes of chromosomal instability (CIN).
  • To summarize the consequences of CIN in various cancer cell lines.
  • To provide practical suggestions for monitoring and controlling CIN in cell culture.

Main Methods:

  • Literature review of studies on chromosomal instability.
  • Analysis of factors contributing to CIN.
  • Synthesis of data on CIN consequences and control measures.

Main Results:

  • Identified key causes of CIN: merotelic attachment, telomere dysfunction, DNA damage response defects, mitotic checkpoint defects, and cell cycle disturbances.
  • Highlighted the impact of CIN on cell line properties and experimental outcomes.
  • Provided a framework for managing CIN in research settings.

Conclusions:

  • Understanding and managing CIN is crucial for improving cancer cell line reproducibility.
  • Implementing monitoring and control strategies can enhance the reliability of research data.
  • This review offers valuable insights for researchers utilizing cancer cell lines.