Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Using known substructures in protein model building and crystallography.

T A Jones, S Thirup

    The EMBO Journal
    |April 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Risk of acute pulmonary embolism in COVID-19 pneumonia compared to community-acquired pneumonia: a retrospective case-control study.

    Clinical radiology·2021
    Same author

    Receptor-mediated exopolysaccharide perception controls bacterial infection.

    Nature·2015
    Same author

    Warfarin dosing after bariatric surgery: a retrospective study of 10 patients previously stable on chronic warfarin therapy.

    Clinical obesity·2015
    Same author

    The effect of stocking density in transit on the carcass quality and welfare of slaughter pigs: 1. Carcass measurements.

    Meat science·2011
    Same author

    Pruning of dendrites and restoration of function after brain damage: Role of the NMDA receptor.

    Restorative neurology and neuroscience·2011
    Same author

    Evaluation of a breast support conveyor to improve poultry welfare on the shackle line.

    The Veterinary record·2011

    This study shows how to build protein models using existing structures. This molecular modeling approach improves accuracy and speed, especially for electron density maps and homologous proteins.

    Area of Science:

    • Structural biology
    • Computational biology
    • Biophysics

    Background:

    • Protein structure determination is crucial for understanding biological function.
    • Challenges exist in molecular modeling, particularly with low-resolution data.
    • Existing protein structures can serve as valuable knowledge bases.

    Purpose of the Study:

    • To develop a novel method for constructing protein models from substructures of unrelated proteins.
    • To enhance molecular modeling accuracy and efficiency, especially for electron density map interpretation and homologous protein modeling.
    • To provide a strategy for building accurate protein models when atomic resolution is unavailable.

    Main Methods:

    • Utilizing a knowledge base of known protein structures for molecular modeling.

    Related Experiment Videos

  • Employing a skeleton representation of electron density maps to improve initial chain tracing.
  • Applying fragment-matching techniques to handle insertions in homologous protein modeling.

    • Main Results:

    • Demonstrated that retinol binding protein can be constructed from substructures of unrelated proteins.
    • Showcased improved accuracy and speed in building models into electron density maps.
    • Successfully bridged gaps for inserted residues during homologous protein modeling.

    Conclusions:

    • The proposed method enables accurate and rapid protein model construction using existing structural information.
    • This approach is particularly beneficial for interpreting low-resolution electron density maps and modeling homologous proteins.
    • Leveraging substructures from diverse proteins offers a powerful strategy in structural bioinformatics.