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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Aug 1, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

High-throughput single-molecule localization microscopy: Potential clinical applications

Wei Shi¹, Shuang Fu¹, Yiming Li¹

¹Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, China.

Clinical and Translational Medicine

|April 25, 2023

Summary

No abstract available in PubMed .

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