Ploidy and proliferative characteristics of sheep epidermal squamous cell carcinoma determined by flow cytometric DNA...

Multiple biopsies from each of 22 primary sheep epidermal squamous cell carcinomas were analysed by flow cytometry to determine the G0/G1 modal DNA content ("ploidy") and cell cycle characteristics within each tumour. Ten of 12 tumours where aneuploidy was present demonstrated uniform intra-tumour aneuploid populations regardless of the site of biopsy. Increasing tumour volume (from stage I/II to stage III/IV lesions) was associated with increased histological variability and ultimate heterogeneity of G0/G1 DNA content, whilst the mean numbers of S phase cells decreased. These features were consistent with the effects of variable tissue hypoxia seen with changes in effective vascularity in developing tumours. Decreasing histological differentiation was associated with an increase in numbers of cells synthesising DNA within 44 biopsies with measurable S phase, and, in stage I/II biopsies, correlated with an increased incidence of aneuploidy.