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Updated: Aug 1, 2025
09:50
Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
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Multiple strategies for the development of multienzyme complex for one-pot reactions
Bahzad Ahmad Farhan1, Li Zhihe2, Shehbaz Ali3
1Institute of Biological Sciences, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan, Pakistan.
Environmental Science and Pollution Research International
|April 25, 2023
Summary
This review explores using multiple enzymes in a one-pot system to efficiently convert wasted biomass into valuable products. This approach offers a sustainable solution for renewable energy and other applications, reducing environmental impact.
Area of Science:
- Biotechnology and Bioengineering
- Sustainable Chemistry
Background:
- Millions of tons of lignocellulosic biomass are wasted annually, contributing to environmental pollution.
- Current methods for biomass conversion are often multi-step, time-consuming, and expensive.
Purpose of the Study:
- To present a strategy utilizing multiple enzymes in a one-pot system for efficient biomass hydrolysis.
- To highlight advancements in enzyme cascade development and immobilization for enhanced reusability.
Main Methods:
- Enzyme cascade arrangement for complete hydrolysis in a single step.
- Immobilization techniques for enzyme reusability under in vitro and in vivo conditions.
- Genetic and metabolic engineering for improving enzyme hydrolytic capacity.
Main Results:
- Demonstration of one-pot multienzyme systems for complete biomass hydrolysis.
- Effective pre-treatment methods (acid/base) enhance enzymatic hydrolysis efficiency.
- Development of recombinant strains with enhanced hydrolytic capabilities.
Conclusions:
- One-pot multienzyme complexes offer a sustainable and efficient method for biomass valorization.
- This technology has broad applications in biofuel, biosensor, medicine, and food industries.
- Enzyme engineering and immobilization are key to optimizing these systems for industrial use.
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