A simple method for the collection of human uterine flushing

A method for the collection of human uterine lavages using a double-barreled cannula that allows for simultaneous injection and aspiration of the flushing is described. The method is simple and quick, is precise, and gives lavage volume recoveries of 77% to 100%. Protein determination of the human uterine flushing gave measurable levels, which are lower than in the plasma. Access to human uterine luminal secretions by this method, which can be repeatedly performed, can facilitate investigations into the sequential biochemical and hormonal uterine secretions in vivo in the same individual.