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Murine model for chronic rhinosinusitis: an interventional study.
Amr F Hamour1, John Jw Lee2, Ewa Wasilewski3
1Department of Otolaryngology - Head and Neck Surgery, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
Summary
A new mouse model effectively mimics chronic rhinosinusitis (CRS) inflammation. This model shows promise for testing new treatments for eosinophilic CRS.
Area of Science:
- Immunology
- Otorhinolaryngology
- Pharmacology
Background:
- Chronic rhinosinusitis (CRS) is a complex sinonasal inflammatory disease.
- Developing targeted treatments requires a reproducible animal model.
- This study optimizes a murine model for eosinophilic CRS.
Purpose of the Study:
- Establish benchmark histological markers for a murine eosinophilic CRS model.
- Validate the model's fidelity in evaluating intranasal treatments.
- Assess the efficacy of mometasone in reversing CRS-induced histopathological changes.
Main Methods:
- Forty-five Balb/c mice underwent a 7-week protocol.
- CRS was induced via ovalbumin (OVA) sensitization and intranasal OVA with Aspergillus oryzae protease.
- Histological analysis and blood counts were performed; CRS mice received saline or mometasone treatment.
Main Results:
- CRS model mice exhibited increased eosinophilic infiltration, hyaline droplets, Charcot-Leyden crystals, and epithelial thickness.
- Mometasone treatment significantly reversed these observed histopathological changes.
- The model successfully replicated key features of eosinophilic CRS.
Conclusions:
- The developed murine model induces significant eosinophilic inflammation in sinonasal mucosa.
- This inflammation is reversible with mometasone treatment.
- The model is suitable for evaluating the efficacy of therapeutics targeting CRS.
