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Functional characterization of hypothetical proteins from Monkeypox virus.

Kajal Gupta1

  • 1Department of Biochemistry, Daulat Ram College, University of Delhi, Delhi, India. gupta.kajal49@gmail.com.

Journal, Genetic Engineering & Biotechnology
|April 26, 2023
PubMed
Summary

This study annotates 3 hypothetical proteins of the Monkeypox virus, identifying roles in apoptosis regulation, viral evasion, and immune response inhibition for drug discovery.

Keywords:
Bioinformatics analysisDrug target identificationHypothetical proteinsMonkeypoxMonkeypox virus

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Area of Science:

  • Virology
  • Bioinformatics
  • Structural Biology

Background:

  • Monkeypox virus (MPXV) is a double-stranded DNA virus causing zoonotic MPX, with recent global spread.
  • The MPXV Zaire-96-I-16 strain genome encodes 191 proteins, including 30 hypothetical proteins with unknown functions.
  • Understanding these hypothetical proteins is crucial for identifying novel drug and vaccine targets against MPX.

Purpose of the Study:

  • To functionally and structurally characterize 30 hypothetical proteins from the MPXV Zaire-96-I-16 strain.
  • To determine physicochemical properties, subcellular localization, and predict functions and structures.
  • To identify potential drug and vaccine targets through bioinformatics analysis.

Main Methods:

  • Utilized various bioinformatics tools for protein characterization.
  • Performed physicochemical property determination, subcellular localization prediction, and functional domain analysis.
  • Employed structure prediction, validation, and analysis, including ligand binding site identification.

Main Results:

  • Successfully assigned confident structure and function to 3 out of 30 hypothetical MPXV proteins.
  • Protein Q8V547 identified as an apoptosis regulator, promoting viral replication.
  • Proteins Q8V4S4 and Q8V4Q4 predicted as nucleases for host immune evasion and NF-kappa-B activation inhibitors, respectively.

Conclusions:

  • Annotated 3 MPXV hypothetical proteins involved in apoptosis regulation, nucleases activity, and NF-kappa-B inhibition.
  • Functional and structural data provide a basis for discovering novel MPX drugs and vaccines via molecular docking.
  • Further in vivo research is recommended to fully elucidate the potential of these annotated proteins.