Simultaneous and sensitive analysis of glyphosate, glufosinate, and their metabolites in surface water by
Takahiro Morimoto1, Akane Ito2, Masaharu Tanimizu3
1Department of Applied Chemistry for Environment, Graduate School of Science and Technology, Kwansei Gakuin University, Gakuen-Uegahara-1, Sanda, Hyogo, 669-1331, Japan. TMorimoto-drc2@kwansei.ac.jp.
Abstract:
Organophosphorus pesticides such as glyphosate and glufosinate are used worldwide, and environmental regulatory values are being adopted in many countries due to their potential toxicity. In the present work, a pretreatment-free analytical method is established in which these two compounds with their metabolites are isolated from each other by anion-exchange HPLC using ammonium acetate (70 mM, pH 3.7) as eluent, and they are detected by triple quadrupole ICP-MS. Very low detection limits of 0.03-0.17 μg L-1 are acquired through the detection of P+ as PO+ via oxygen reaction mode, and quantitative recovery was obtained from the spike-recovery test on river water samples containing phosphate ion as an isobaric interferent. In addition, a constant sensitivity per molar concentration was achieved regardless of the compounds due to the powerful ion source of ICP-MS. This property suggests that semi-quantitative analysis of unknown P-bearing compounds is possible from one calibration curve.
