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Articles linked to this work by shared authors, journal, and citation graph.

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Selective feeding behavior of Spissistilus festinus (Hemiptera: Membracidae) among Fabaceae and Vitaceae plants identified by choice assays.

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[Grapevine fanleaf virus biology].

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Profiling Plant Proteome and Transcriptome Changes during Grapevine Fanleaf Virus Infection.

Brandon G Roy1, Stacy DeBlasio2, Yong Yang2

  • 1Plant Pathology and Plant-Microbe Biology Section, Cornell AgriTech at the New York State Agricultural Experiment Station, Cornell University, Geneva, New York 14456, United States.

Journal of Proteome Research
|April 26, 2023
PubMed
Summary

A single amino acid change in grapevine fanleaf virus (GFLV) RNA-dependent RNA polymerase (RdRP) alters plant proteome and transcriptome, causing transient vein clearing symptoms in Nicotiana benthamiana.

Keywords:
Nicotiana benthamianaRNA-dependent RNA polymerasegrapevinenepovirusproteomicssymptomologytime-coursetranscriptomicsvirus

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Area of Science:

  • Plant Virology
  • Molecular Plant-Pathogen Interactions
  • Systems Biology

Background:

  • Plant viruses induce diverse symptoms, but the molecular mechanisms are not fully understood.
  • Understanding host responses to viral infection is crucial for developing disease-resistant crops.

Purpose of the Study:

  • To investigate host proteome and transcriptome changes in Nicotiana benthamiana infected with grapevine fanleaf virus (GFLV).
  • To identify host pathways involved in viral symptom development, specifically vein clearing.
  • To elucidate the role of a single amino acid in the viral RNA-dependent RNA polymerase (RdRP) in mediating these changes.

Main Methods:

  • Comparative, time-course proteomic analysis using liquid chromatography tandem mass spectrometry.
  • Comparative, time-course transcriptomic analysis using 3' ribonucleic acid sequencing.
  • Analysis of plants infected with wildtype GFLV strains (symptomatic and asymptomatic) and their asymptomatic RdRP mutant strains.

Main Results:

  • At 7 days post-inoculation (dpi) during peak vein clearing, host protein and gene ontologies related to immune response, gene regulation, and secondary metabolite production were overrepresented.
  • At 4 dpi (pre-symptom) and 12 dpi (post-symptom), ontologies related to chitinase activity, hypersensitive response, and transcriptional regulation were identified.
  • A single amino acid change in GFLV RdRP significantly altered the host proteome (~1%) and transcriptome (~8.5%) associated with transient vein clearing.

Conclusions:

  • A single amino acid in the viral RdRP is critical for modulating host responses and inducing transient symptoms.
  • This study highlights the complex interplay between viral components and host pathways during infection.
  • The findings provide insights into the virus-host arms race at the molecular level.