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Updated: Aug 1, 2025

Measurements of Physiological Stress Responses in C. Elegans
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Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

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Casein kinase 1 gamma regulates oxidative stress response via interacting with the NADPH dual oxidase complex

Yiman Hu1, Zhaofa Xu1, Qian Pan1

  • 1Center for Medical Genetics, School of Life Sciences, Central South University, Changsha, Hunan, China.

Plos Genetics
|April 26, 2023
PubMed

Insights

Casein kinase 1 gamma (CSNK-1) regulates oxidative stress and reactive oxygen species (ROS) levels in C. elegans. This finding reveals a conserved mechanism for maintaining ROS homeostasis.

Area of Science:

  • Cellular Biology
  • Genetics
  • Biochemistry

Background:

  • Oxidative stress is a critical biological process involving conserved regulatory mechanisms.
  • Key regulators of oxidative stress response and reactive oxygen species (ROS) levels are not fully understood.

Purpose of the Study:

  • To identify novel regulators of oxidative stress response and ROS homeostasis.
  • To investigate the role of C. elegans casein kinase 1 gamma (CSNK-1) in oxidative stress.

Main Methods:

  • Genetic nonallelic noncomplementation screens in C. elegans.
  • Biochemical interaction assays.
  • ROS level measurements in C. elegans and human cells.
  • Nrf2 (SKN-1) interaction studies.

Main Results:

  • CSNK-1 plays a novel role in regulating oxidative stress and ROS levels in C. elegans.
  • CSNK-1 genetically interacts with NADPH dual oxidase genes (bli-3/tsp-15/doxa-1).
  • Biochemical interactions were observed between CSNK-1 and DOXA-1, and their human orthologs.
  • CSNK-1 is essential for normal ROS levels, and its human orthologs can modulate ROS.
  • CSNK-1 interacts with Nrf2 (SKN-1) in oxidative stress response.

Conclusions:

  • CSNK-1 (CSNK1G) represents a novel conserved regulator of ROS homeostasis.
  • This discovery provides new insights into the fundamental mechanisms of oxidative stress response.

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