Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Viral Replication: Lytic Cycle01:20

Viral Replication: Lytic Cycle

98
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
98
Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

72
Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
72
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

60
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
60
Viral Replication: Lysogenic Cycle01:16

Viral Replication: Lysogenic Cycle

94
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
94
DNA Bacteriophages01:26

DNA Bacteriophages

69
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
69
Retrovirus Life Cycles01:10

Retrovirus Life Cycles

46.3K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
46.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid tests for Bundibugyo virus in DR Congo.

Lancet (London, England)·2026
Same author

Retraction Note: Mechanism of HCV's resistance to IFN-α in cell culture involves expression of functional IFN-α receptor 1.

Virology journal·2026
Same author

Searching for immune correlates in Lassa vaccine development - workshop report.

NPJ vaccines·2026
Same author

Flush With Data (or) Optimizing and Validating the Efficacy of Free and Computationally Simple 16S Metabarcoding Approaches for Use in Wastewater Surveillance.

Environmental microbiology·2026
Same author

Evaluation of candidate reference materials for the harmonization of Lassa fever serology.

Emerging microbes & infections·2026
Same author

Evaluation of antigen and antibody assays for surveillance of Lassa virus in peri-domestic rodents in southeastern Sierra Leone: a cross-sectional study.

The Lancet. Microbe·2026
Same journal

Resolution Biology in Soft Tissue Joint Disease.

Current topics in microbiology and immunology·2026
Same journal

A 25+ Year Journey on Yeast-Regulated Cell Death Research.

Current topics in microbiology and immunology·2026
Same journal

Adoptive T-Cell Immunotherapy.

Current topics in microbiology and immunology·2026
Same journal

Resolution Pharmacology Targeting the Melanocortin System.

Current topics in microbiology and immunology·2026
Same journal

Resolution of Skeletal Muscle Inflammation: Role of Specialized Pro-resolving Lipid Mediators in the Recovery from Exercise, Injury, and Disease.

Current topics in microbiology and immunology·2026
Same journal

Epstein-Barr Virus: From the Detection of Sequence Polymorphisms to the Recognition of Viral Strains.

Current topics in microbiology and immunology·2026
See all related articles

Related Experiment Video

Updated: Aug 1, 2025

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes
10:11

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Published on: September 27, 2014

36.3K

Lassa Virus Structural Biology and Replication.

Robert F Garry1,2,3

  • 1School of Medicine, Department of Microbiology and Immunology, Tulane University, New Orleans, LA, 70112, USA. rfgarry@tulane.edu.

Current Topics in Microbiology and Immunology
|April 26, 2023
PubMed
Summary
This summary is machine-generated.

Lassa virus causes Lassa fever, a deadly hemorrhagic fever in West Africa. Understanding its structure and replication aids in developing vaccines and drugs.

More Related Videos

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
06:02

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

6.9K
Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
11:48

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Published on: April 4, 2019

15.9K

Related Experiment Videos

Last Updated: Aug 1, 2025

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes
10:11

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Published on: September 27, 2014

36.3K
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
06:02

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

6.9K
Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
11:48

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Published on: April 4, 2019

15.9K

Area of Science:

  • Virology
  • Molecular Biology
  • Public Health

Background:

  • Lassa virus (LASV) causes Lassa fever, a severe hemorrhagic illness endemic to West Africa.
  • LASV is an enveloped virus with a single-stranded RNA genome encoding key proteins like nucleoprotein, glycoprotein, zinc, and large polymerase.
  • Viral entry involves a clathrin-independent pathway using specific cellular receptors.

Purpose of the Study:

  • To provide a comprehensive overview of Lassa virus structure, replication, and entry mechanisms.
  • To highlight recent advances in understanding LASV biology.
  • To underscore the implications of this knowledge for therapeutic development.

Main Methods:

  • Review of existing literature on Lassa virus structural biology and replication.
  • Analysis of viral genome organization and protein functions.
  • Examination of the endocytic pathway utilized by LASV for cell entry.

Main Results:

  • Detailed description of LASV genome segments and encoded proteins (nucleoprotein, glycoprotein, zinc, large polymerase).
  • Elucidation of the clathrin-independent endocytic entry pathway involving alpha-dystroglycan and LAMP1.
  • Identification of key viral components essential for replication and pathogenesis.

Conclusions:

  • Advances in understanding LASV biology are crucial for combating Lassa fever.
  • Knowledge of viral structure and replication facilitates the development of novel vaccines and antiviral drugs.
  • Continued research is vital for controlling the spread and impact of Lassa fever in endemic regions.