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Possible mitigating effect of adropin on lung injury in diabetic rats: Targeting the role of Rho A/Rho-associated
Fatma H Rizk1, Mervat H El-Saka1, Rowida Raafat Ibrahim2
1Department of Physiology, Faculty of Medicine, Tanta University, Egypt.
Abstract:
This study evaluated possible mitigating effect of adropin on lung injury in diabetic rats, targeting role of Rho A/Rho-associated kinase pathway. Rats were allocated into four groups: control, adropin, diabetic, and diabetic+adropin groups. At the termination of the experiment, serum fasting glucose, insulin and adropin levels and insulin resistance were calculated. Wet/dry ratio, histopathological, immunohistochemical analyses, and relative real time gene expression of lung tissue was determined. Interleukin-6, tumor necrosis factor alpha, malondialdehyde, 8-Oxo-2'-deoxyguanosine, reduced glutathione, superoxide dismutase, Bcl-2, BAX, myeloperoxidase, intracellular adhesion molecule-1, vascular cell adhesion molecule-1, and transforming growth factor-β were determined in lung tissue. Adropin treatment in diabetic rats notably attenuated hyperglycemia and insulin resistance. Also, it mitigated diabetic lung injury via suppressing effect on Rho A/ROCK pathway, apoptosis, inflammatory reactions, oxidative stress, and fibrosis of lung tissue. Adropin can be considered as a promising therapeutic agent for treating diabetic lung injury.
Insights
Adropin treatment improved hyperglycemia and insulin resistance in diabetic rats. It also protected against diabetic lung injury by reducing inflammation, oxidative stress, and fibrosis via the Rho A/ROCK pathway.
Area of Science:
- Endocrinology and Metabolism
- Pulmonology
- Molecular Biology
Background:
- Diabetes mellitus is associated with increased risk of lung complications.
- The Rho A/Rho-associated kinase (ROCK) pathway plays a role in various cellular processes, including inflammation and fibrosis, which may contribute to diabetic lung injury.
Purpose of the Study:
- To investigate the potential protective effects of adropin against lung injury in a rat model of diabetes.
- To explore the role of the Rho A/ROCK pathway in adropin's mitigating effects on diabetic lung injury.
Main Methods:
- Rats were divided into control, adropin, diabetic, and diabetic+adropin groups.
- Biochemical parameters (glucose, insulin, adropin, insulin resistance) and lung tissue analyses (histopathology, gene expression, inflammatory markers, oxidative stress markers, apoptosis markers, fibrosis markers) were performed.
Main Results:
- Adropin treatment significantly reduced hyperglycemia and insulin resistance in diabetic rats.
- Adropin administration attenuated lung injury by suppressing the Rho A/ROCK pathway, apoptosis, inflammation, oxidative stress, and fibrosis in lung tissue.
Conclusions:
- Adropin demonstrates a mitigating effect on diabetic lung injury.
- Adropin may represent a promising therapeutic strategy for managing lung complications in diabetic patients, potentially through modulation of the Rho A/ROCK pathway.
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