Age related changes and sex related differences of functional brain networks in childhood: A high-density EEG study

The aim of this study was to explore functional network age-related changes and sex-related differences during the early lifespan with a high-density resting state electroencephalography (rs-EEG).

We analyzed two data sets of high-density rs-EEG in healthy children and adolescents. We recorded a 64-channel EEG and calculated functional connectomes in 27 participants aged 5-18 years. To validate our results, we used publicly available data and calculated functional connectomes in another 86 participants aged 6-18 years from a 128-channel rs-EEG. We were primarily interested in alpha frequency band, but we also analyzed theta and beta frequency bands.

We observed age-related increase of characteristic path, clustering coefficient and interhemispheric strength in the alpha frequency band of both data sets and in the beta frequency band of the larger validation data set. Age-related increase of global efficiency was seen in the theta band of the validation data set and in the alpha band of the test data set. Increase in small worldness was observed only in the alpha frequency band of the test data set. We also observed an increase of individual peak alpha frequency with age in both data sets. Sex-related differences were only observed in the beta frequency band of the larger validation data set, with females having higher values than same aged males.

Functional brain networks show indices of higher segregation, but also increasing global integration with maturation. Age-related changes are most prominent in the alpha frequency band.

To the best of our knowledge, our study was the first to analyze maturation related changes and sex-related differences of functional brain networks with a high-density EEG and to compare functional connectomes generated from two diverse high-density EEG data sets. Understanding the age-related changes and sex-related differences of functional brain networks in healthy children and adolescents is crucial for identifying network abnormalities in different neurologic and psychiatric conditions, with the aim to identify possible markers for prognosis and treatment.