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Protective effects of resolvin D1 in Pseudomonas aeruginosa keratitis
Jiayin Wu1, Jianlu Gao2, Lili Yi3
1Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan, Shandong 250014, China; Department of Ophthalmology, Liaocheng People's Hospital, Liaocheng, Shandong 252000, China.
Purpose:
Here, we explored the protective effects of resolvin D1 (RvD1) in Pseudomonas aeruginosa (PA) keratitis.
Methods:
C57BL/6 (B6) mice were used as an animal model of PA keratitis. Plate counting and clinical scores were used to assess the severity of the infection and the therapeutic effects of RvD1 in the model. Myeloperoxidase assay was used to detect neutrophil infiltration and activity. Quantitative PCR (qPCR) was used to examine the expression of proflammatory and anti-inflammatory mediators. Immunofluorescence staining and qPCR were performed to identify macrophage polarization.
Results:
RvD1 treatment alleviated PA keratitis severity by decreasing corneal bacterial load and inhibiting neutrophil infiltration in the mouse model. Furthermore, RvD1 treatment decreased mRNA levels of TNF-α, IFN-γ, IL-1β, CXCL1, and S100A8/9 while increasing those of IL-1RA, IL-10, and TGF-β1. RvD1 treatment also reduced the aggregation of M1 macrophages and increased that of M2 macrophages. RvD1 provided an auxiliary effect in gatifloxacin-treated mice with PA keratitis.
Conclusion:
Based on these findings, RvD1 may improve the prognosis of PA keratitis by inhibiting neutrophil recruitment and activity, dampening the inflammatory response, and promoting M2 macrophage polarization. Thus, RvD1 may be a potential complementary therapy for PA keratitis.
Insights
Resolvin D1 (RvD1) reduces Pseudomonas aeruginosa (PA) keratitis severity by decreasing bacterial load and inflammation. This compound may serve as a complementary therapy for PA keratitis.
Area of Science:
- Ophthalmology
- Infectious Diseases
- Immunology
Background:
- Pseudomonas aeruginosa (PA) keratitis is a serious eye infection.
- Effective treatments are crucial for preventing vision loss.
Purpose of the Study:
- To investigate the protective effects of resolvin D1 (RvD1) in a mouse model of PA keratitis.
- To assess RvD1's impact on infection severity, inflammation, and immune cell activity.
Main Methods:
- Utilized a C57BL/6 mouse model of PA keratitis.
- Assessed therapeutic effects using plate counts, clinical scores, myeloperoxidase assays, and quantitative PCR (qPCR).
- Examined macrophage polarization via immunofluorescence staining and qPCR.
Main Results:
- RvD1 treatment significantly alleviated PA keratitis severity.
- RvD1 decreased corneal bacterial load and neutrophil infiltration.
- RvD1 modulated pro-inflammatory and anti-inflammatory mediator expression and promoted M2 macrophage polarization.
Conclusions:
- RvD1 demonstrates protective effects against PA keratitis.
- RvD1 may improve outcomes by inhibiting neutrophil activity, reducing inflammation, and promoting M2 macrophages.
- RvD1 shows potential as an adjunctive therapy for PA keratitis.
