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Related Experiment Video

Updated: Aug 1, 2025

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
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Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

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Characterizing the function-related specific assembly pattern of matrix metalloproteinase-14 by dSTORM imaging.

Dian Ge1, Junling Chen1, Zhiyong Zhao1

  • 1Improve-WUST Joint Laboratory of Advanced Technology for Point-of-Care Testing and Precision Medicine, School of Chemistry & Chemical Engineering, Wuhan University of Science and Technology, 947 Heping Street, Wuhan, Hubei, 430081, China.

Talanta
|April 27, 2023
PubMed
Summary

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Matrix metalloproteinase-14 (MMP14) aggregates on cell membranes, coordinating cell migration. Its clustering increases with hydrolysis efficiency and co-localization with substrate PTK7 on highly migratory cells.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Matrix metalloproteinase-14 (MMP14) is a transmembrane enzyme regulating cell migration and cancer metastasis.
  • Understanding MMP14's single-molecule function requires detailed knowledge of its spatial organization.

Purpose of the Study:

  • To characterize the single-molecule distribution and spatial assembly of MMP14 on cell membranes.
  • To investigate the relationship between MMP14 localization, migratory capacity, and substrate interaction.

Main Methods:

  • Development and application of aptamer probes for MMP14 labeling.
  • Utilizing direct Stochastic Optical Reconstruction Microscopy (dSTORM) for high-resolution imaging.
  • Analyzing MMP14 co-localization with its substrate PTK7.
Keywords:
Cell migrationClusterDirect stochastic optical reconstruction microscopyMMP14Single molecule

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Related Experiment Videos

Last Updated: Aug 1, 2025

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
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Published on: March 6, 2018

10.9K
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
08:43

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

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Detection of Functional Matrix Metalloproteinases by Zymography
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Detection of Functional Matrix Metalloproteinases by Zymography

Published on: November 8, 2010

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Main Results:

  • MMP14 predominantly forms clusters on cell membranes.
  • MMP14 clustering is more pronounced in high-migratory cells and correlates with enhanced hydrolysis efficiency.
  • Significant co-localization of MMP14 with PTK7 was observed, decreasing as cell migration weakens.

Conclusions:

  • MMP14 spatial organization, particularly its clustering and co-localization with PTK7, is crucial for coordinating cell migration.
  • This study provides insights into MMP14's role in cell migration and cancer metastasis at the single-molecule level.