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Related Concept Videos

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Centrifugation

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Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
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The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
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Updated: Aug 1, 2025

Sperm Collection of Differential Quality Using Density Gradient Centrifugation
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Defining an Optimal Range of Centrifugation Parameters for Canine Semen Processing.

Nicole Sugai1, Stephen Werre2, Julie Cecere1

  • 1Department of Small Animal Clinical Sciences, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA 24061, USA.

Animals : an Open Access Journal From MDPI
|April 28, 2023
PubMed
Summary

Centrifugation parameters for canine semen processing were evaluated. Results show that 400-900 g for 5-10 minutes effectively recovers sperm without compromising quality during cooling.

Keywords:
centrifugationchilledcooleddogmorphologymotilityspermviability

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Area of Science:

  • Veterinary Medicine
  • Reproductive Biology
  • Animal Science

Background:

  • Canine semen processing for artificial insemination requires optimal centrifugation parameters.
  • Understanding the impact of centrifugation force and duration on semen quality is crucial for successful reproductive outcomes.

Purpose of the Study:

  • To determine clinically relevant centrifugation parameters (gravitational force and time) for processing canine semen.
  • To evaluate the effect of different centrifugation settings on spermatozoa recovery rate and semen quality, including plasma membrane integrity, motility, and morphology.
  • To assess the long-term effects of semen processing and cooling on sperm quality.

Main Methods:

  • Semen from 14 healthy dogs was processed using six centrifugation groups (400, 720, 900 g for 5 or 10 min).
  • Spermatozoa recovery rate (RR) was calculated post-centrifugation.
  • Plasma membrane integrity, total and progressive motility, and morphology were assessed at multiple time points (T0, T1, T2, T3) before and after cooling.

Main Results:

  • Spermatozoa recovery rates were consistently high (>98%) and not significantly different across centrifugation groups.
  • Plasma membrane integrity and motility declined significantly during cooling, but these effects were independent of centrifugation parameters.
  • Centrifugation parameters did not significantly impact semen quality metrics at any assessed time point.

Conclusions:

  • Centrifugation settings ranging from 400 g to 900 g for 5 to 10 minutes are appropriate for processing canine semen.
  • The chosen centrifugation parameters do not adversely affect canine sperm quality during processing or subsequent cooling.
  • Cooling poses a greater challenge to canine sperm quality than the tested centrifugation protocols.