Related Experiment Video

Updated: Aug 1, 2025

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
08:57

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Published on: February 24, 2018

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Antagonistic Interactions in Mitochondria ROS Signaling Responses to Manganese

Jolyn Fernandes1,2, Karan Uppal2, Ken H Liu2

  • 1Section of Neonatal-Perinatal Medicine, Department of Pediatrics, College of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

Antioxidants (Basel, Switzerland)
|April 28, 2023
PubMed

Insights

Antagonistic interactions, where a single agent causes opposing signals, are a generalized cell response to mitochondrial reactive oxygen species (ROS) signaling. This study reveals connections between cellular functions and these complex signaling pathways.

Area of Science:

  • Cellular biology
  • Systems biology
  • Biochemistry

Background:

  • Antagonistic interactions involve opposing beneficial and adverse signaling from a single agent.
  • Understanding these interactions is crucial for addressing pathologies caused by harmful agents or failed beneficial mechanisms.

Purpose of the Study:

  • To investigate opposing cellular responses at a systems level.
  • To explore the relationship between gene expression, metabolite changes, and mitochondrial function.

Main Methods:

  • Utilized a transcriptome-metabolome-wide association study (TMWAS).
  • Incorporated measurements of mitochondrial oxidative stress (mtOx) and oxygen consumption rate (mtOCR).
  • Analyzed cells with varying manganese (Mn) concentrations.

Main Results:

  • Adverse neuroinflammatory and fatty acid metabolism signaling linked to mtOx.
  • Beneficial ion transport and neurotransmitter metabolism linked to mtOCR.
  • Identified opposing transcriptome-metabolome interactions within cellular communities, connected to biological functions.

Conclusions:

  • Antagonistic interaction is a generalized cellular system response to mitochondrial ROS signaling.
  • TMWAS provides a systems-level readout of complex cellular signaling pathways.
Keywords:
antioxidantsexposomeintegrated omicsmitochondrianetwork of genes and metabolitesneurotoxicantoxidative phosphorylationredox regulation

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