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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Homologous Recombination02:31

Homologous Recombination

50.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Related Experiment Video

Updated: Aug 1, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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The CRISPR/Cas System: A Customizable Toolbox for Molecular Detection.

Yuxuan He1, Wei Yan1, Likun Long1

  • 1Institute of Agricultural Quality Standard and Testing Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, China.

Genes
|April 28, 2023
PubMed
Summary
This summary is machine-generated.

Clustered regularly interspaced short palindromic repeats (CRISPR) and Cas systems offer precise genome analysis. Optimizing these systems enhances their sensitivity and accuracy for molecular diagnostics.

Keywords:
CRISPR/Cas systemapplicationmolecular detectionpoint-of-care testing

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • CRISPR-Cas systems are powerful tools for genome analysis due to specificity and programmability.
  • Current limitations in CRISPR-Cas systems necessitate integration with amplification or signal detection methods for optimal performance.
  • CRISPR-Cas technology holds potential as a highly sensitive and accurate biosensing platform.

Purpose of the Study:

  • To review the molecular characteristics and application value of CRISPR-Cas systems in molecular detection.
  • To explore recent research progress and future development directions for CRISPR-Cas based detection.
  • To provide a theoretical foundation for advancing CRISPR-Cas molecular detection technologies.

Main Methods:

  • Focus on the principle, performance, and method development challenges of CRISPR-Cas systems.
  • Review strategies for designing molecular detection platforms using CRISPR-Cas.
  • Discuss optimization of reaction components and conditions for enhanced detection.

Main Results:

  • CRISPR-Cas systems exhibit high specificity, programmability, and multi-system compatibility for nucleic acid recognition.
  • Performance optimization, signal enhancement, and reaction system compatibility are key strategies for CRISPR-Cas based detection.
  • Integration with other techniques and optimized conditions are crucial for maximizing detection performance.

Conclusions:

  • CRISPR-Cas systems are evolving into ultra-sensitive, convenient, and accurate biosensing platforms.
  • Further development requires addressing challenges in principle, performance, and method development.
  • This review provides a foundation for the application of CRISPR-Cas systems in advanced molecular detection.