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Related Experiment Video

Updated: Aug 1, 2025

Gene Expression Analyses in Human Follicles
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RNA Transcripts in Human Ovarian Cells: Two-Time Cryopreservation Does Not Affect Developmental Potential.

Yang Zhou1, Wanxue Wang1, Plamen Todorov2

  • 1Department of Obstetrics and Gynecology, Medical Faculty, Cologne University, 50931 Cologne, Germany.

International Journal of Molecular Sciences
|April 28, 2023
PubMed
Summary
This summary is machine-generated.

Repeatedly cryopreserving ovarian tissue does not significantly impact cell developmental potential. This finding supports re-freezing thawed ovarian tissue for future transplantation when immediate use is medically impossible.

Keywords:
DEGKEGG pathway and PPI analysiscellscryopreservationdevelopmentfolliclesgene ontologyhumanmRNA sequencingovarian

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Area of Science:

  • Reproductive biology
  • Cryobiology
  • Molecular biology

Background:

  • Ovarian tissue cryopreservation is vital for fertility preservation.
  • Repeated cryopreservation protocols for ovarian tissue are not well-established.
  • Understanding molecular changes during repeated cryopreservation is crucial for clinical application.

Purpose of the Study:

  • To investigate the effects of repeated cryopreservation on ovarian tissue gene expression and cellular functions.
  • To analyze gene and protein interactions following one-time versus two-time cryopreservation.
  • To assess the viability of follicles for artificial ovary formation after repeated cryopreservation.

Main Methods:

  • Utilized second-generation mRNA sequencing to analyze transcriptome profiles of four groups: one-time cryopreserved, two-time cryopreserved, one-time cryopreserved and cultured, and two-time cryopreserved and cultured.
  • Evaluated morphological and biological activity of primordial, primary, and secondary follicles.
  • Performed gene function annotation and protein-protein interaction analysis.

Main Results:

  • Two-time cryopreservation did not significantly alter the developmental potential of ovarian cells.
  • Identified the CEBPB/CYP19A1 pathway's role in estrogen activity and CD44's importance in ovarian cell development.
  • Observed minor morphological and biological activity changes in follicles, with retained potential for artificial ovary formation.

Conclusions:

  • Repeated cryopreservation of ovarian tissue is a viable option when transplantation is not immediately feasible.
  • The molecular mechanisms, including specific gene pathways, are largely unaffected by a second round of cryopreservation.
  • Ovarian follicles retain developmental potential for artificial ovary construction after repeated cryopreservation.