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RNA-Dependent RNA Targeting by CRISPR-Cas Systems: Characterizations and Applications
Natalia Gunitseva1, Marta Evteeva1, Anna Borisova1
1Complex of NBICS Technologies, National Research Center "Kurchatov Institute", 123182 Moscow, Russia.
International Journal of Molecular Sciences
|April 28, 2023
Summary
RNA-targeting CRISPR-Cas systems offer precise gene expression control without complete elimination. These versatile systems are revolutionizing biosensing, diagnostics, and gene editing applications.
Area of Science:
- Molecular Biology
- Biotechnology
- Genetics
Background:
- Current genome editing technologies significantly impact molecular biology, medicine, and biotechnology.
- RNA-targeting presents a novel approach for spatiotemporal gene expression control without complete gene elimination.
- CRISPR-Cas RNA-targeting systems have redefined biosensing and enabled new applications.
Purpose of the Study:
- To review the current state of CRISPR-Cas systems that target and cleave RNA.
- To summarize the diverse potential applications of RNA-targeting systems.
Main Methods:
- Literature review of CRISPR-Cas systems targeting RNA.
- Analysis of RNA-targeting mechanisms and applications.
Main Results:
- Identification of specific CRISPR-Cas systems capable of binding and cleaving RNA substrates.
- Overview of applications including genomic editing, virus diagnostics, biomarkers, and transcription regulation.
Conclusions:
- RNA-targeting CRISPR-Cas systems provide a powerful tool for precise gene expression modulation.
- These systems hold significant promise for advancing various fields, from diagnostics to biotechnology.
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