Related Experiment Video

Updated: Aug 1, 2025

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
08:19

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Published on: July 20, 2019

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Tumor Necrosis Factor Superfamily 14 (LIGHT) Restricts Neovascularization by Decreasing Circulating Endothelial

Chien-Yi Hsu1,2, Chun-Yao Huang1,2,3, Chun-Ming Shih1,2

  • 1Taipei Heart Institute and Division of Cardiology, Department of Internal Medicine, Taipei Medical University, Taipei 110, Taiwan.

International Journal of Molecular Sciences
|April 28, 2023
PubMed

Insights

Tumor necrosis factor superfamily 14 (LIGHT) inhibits angiogenesis in ischemic tissues. This protein impairs endothelial progenitor cell function, hindering blood vessel formation after injury.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Tumor necrosis factor superfamily 14 (TNFSF14), also known as LIGHT, has diverse physiological roles.
  • LIGHT's impact on angiogenesis following tissue ischemia remains largely uncharacterized.
  • Understanding LIGHT's function in ischemia is crucial for developing therapeutic strategies.

Purpose of the Study:

  • To investigate the role of LIGHT in angiogenesis after tissue ischemia.
  • To elucidate the underlying mechanisms of LIGHT's effects on endothelial progenitor cells (EPCs).

Main Methods:

  • Hind limb ischemia model in C57BL/6 mice.
  • Doppler ultrasound, immunohistochemistry, and Western blotting for angiogenesis analysis.
  • In vitro studies using human EPCs to assess cellular functions and signaling pathways.

Main Results:

  • LIGHT injection significantly inhibited angiogenesis in ischemic limbs in vivo.
  • In vitro, LIGHT reduced EPC migration, tube formation, mitochondrial respiration, and promoted senescence.
  • LIGHT negatively impacted the Akt signaling pathway, eNOS, and mitochondrial respiration in EPCs.

Conclusions:

  • LIGHT demonstrably inhibits angiogenesis in the context of tissue ischemia.
  • Impaired EPC function, mediated by LIGHT, is a key factor in this inhibition.
  • LIGHT may represent a therapeutic target for managing ischemic conditions.
Keywords:
LIGHTendothelial progenitor cellstumor necrosis factor superfamily 14

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