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Fully Aqueous Self-Assembly of a Gold-Nanoparticle-Based Pathogen Sensor.

Timothy Robson1, Deepan S H Shah2, Rebecca J L Welbourn3

  • 1Biosciences Institute, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.

International Journal of Molecular Sciences
|April 28, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a low-cost method for detecting viruses using gold nanoparticle (AuNP) assemblies. This localized surface plasmon resonance (LSPR) biosensor is suitable for rapid clinical sample analysis.

Keywords:
AAPTMSLSPRPEDAgold nanoparticleinfluenzanucleoproteinouter membrane proteinprotein engineeringscFVsilanization

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Area of Science:

  • Nanotechnology
  • Biomolecular Engineering
  • Biosensing

Background:

  • Surface plasmon resonance (SPR) is a sensitive technique for studying biomolecular interactions but is often cost-prohibitive for routine clinical diagnostics.
  • Existing methods for biosensor development can be complex and expensive, limiting their widespread application.

Purpose of the Study:

  • To develop a simplified, cost-effective method for creating virus-detecting biosensors using gold nanoparticle (AuNP) assemblies.
  • To demonstrate the formation and characterization of protein sensor layers on AuNPs for detecting specific biomolecules.

Main Methods:

  • Fabrication of AuNP assemblies on silanized glass surfaces using aqueous buffers at room temperature.
  • Utilizing localized surface plasmon resonance (LSPR) to monitor the assembly of protein scaffolds on AuNPs.
  • Employing neutron reflectometry to analyze the structure of the biological layer on AuNPs.
  • Integrating in vitro-selected single-chain antibody (scFv)-membrane protein fusions as artificial receptor layers within AuNP-coated glass capillaries.

Main Results:

  • Successfully formed AuNP assemblies on glass substrates, exhibiting distinct LSPR absorbance peaks.
  • Demonstrated the self-assembly of oriented protein sensor layers on AuNPs.
  • Validated the function of artificial flu sensor layers using LSPR in a capillary-based system.
  • Showcased a simplified, low-cost approach for creating specific protein sensors.

Conclusions:

  • This work presents a straightforward method for constructing oriented protein sensor arrays on nanostructured surfaces.
  • The developed biosensor platform offers a cost-effective alternative for clinical sample analysis, utilizing artificial receptor proteins.
  • The approach simplifies sensor fabrication and enables rapid production of low-cost, highly specific sensor proteins.