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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Studying the Cytoskeleton01:17

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The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
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Mechanical Protein Functions01:58

Mechanical Protein Functions

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Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force. 
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Related Experiment Video

Updated: Aug 1, 2025

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
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Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

Published on: June 27, 2013

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Insights in Cell Biomechanics through Atomic Force Microscopy.

Sajedeh Kerdegari1, Paolo Canepa1, Davide Odino1

  • 1Dipartimento di Fisica, Università di Genova, Via Dodecaneso 33, 16146 Genova, Italy.

Materials (Basel, Switzerland)
|April 28, 2023
PubMed
Summary

Atomic Force Microscopy (AFM) advances cell/tissue mechanics and adhesion studies. This review highlights AFM

Keywords:
AFMcell mechanical propertiescellsmechanobiology

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Area of Science:

  • Biophysics
  • Cell Biology
  • Biotechnology

Background:

  • Mechanobiology is crucial in biomedical and biotechnological fields.
  • Atomic Force Microscopy (AFM) provides high force sensitivity and spatial resolution for biological samples.
  • AFM enables detailed investigation of cellular and tissue mechanical properties.

Purpose of the Study:

  • To review recent advances in AFM-based cell/tissue mechanics and adhesion research.
  • To critically discuss AFM applications in mechanobiology over the past decade.
  • To explore AFM's role in understanding disease mechanisms and diagnostics.

Main Methods:

  • Utilizing Atomic Force Microscopy (AFM) for force measurements and spatial mapping.
  • Analyzing cellular mechanosensing and adaptation to mechanical environments.
  • Investigating cell adhesion at single-cell and quantitative levels.

Main Results:

  • AFM allows subcellular resolution mechanical mapping of biological samples.
  • AFM contributes to characterizing pathological mechanisms in cancer and neurodegenerative diseases.
  • AFM facilitates the development of cell mechanics-based diagnostic tools and biomarkers.

Conclusions:

  • AFM is a powerful tool for advancing mechanobiology research.
  • AFM insights into cell mechanics and adhesion are vital for understanding diseases.
  • AFM holds significant potential for novel diagnostic and therapeutic strategies.