Related Experiment Video
Updated: Aug 1, 2025
06:27
Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells
Published on: May 6, 2013
16.8K
Development of Type 1 Diabetes in Mice Is Associated with a Decrease in IL-2-Producing ILC3 and FoxP3+ Treg in the
Tamara Saksida1, Verica Paunović2, Ivan Koprivica1
1Department of Immunology, Institute for Biological Research "Siniša Stanković"-National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.
Molecules (Basel, Switzerland)
|April 28, 2023
Summary
Type 1 diabetes (T1D) progression is linked to fewer IL-2-producing innate lymphoid cells type 3 (ILC3) and regulatory T cells (Treg) in the gut. Antibiotic use worsened T1D and reduced these crucial immune cells.
Area of Science:
- Immunology
- Endocrinology
- Gastroenterology
Background:
- Type 1 diabetes (T1D) is an autoimmune disease linked to gut immunity.
- Regulatory T cells (Treg) are crucial for preventing autoimmunity.
- Type 3 innate lymphoid cells (ILC3) maintain Treg in the gut.
Purpose of the Study:
- To investigate the relationship between ILC3 and Treg in T1D pathogenesis.
- To explore the role of ILC3-Treg axis in T1D development.
Main Methods:
- Studied NOD mice and streptozotocin-induced T1D models.
- Analyzed frequencies of ILC3 and Treg in small intestine lamina propria (SILP).
- Assessed impact of antibiotic treatment on T1D incidence and immune cell populations.
Main Results:
- Diabetic mice showed reduced frequencies of IL-2-producing ILC3 and Treg in SILP.
- Antibiotic treatment increased T1D incidence and decreased IL-2+ ILC3 and Treg in SILP.
- Lower IL-2-expressing ILC3 and Treg correlated with diabetes progression.
Conclusions:
- Reduced IL-2-producing ILC3 and Treg in SILP are associated with T1D development and severity.
- The ILC3-Treg axis in the gut may play a significant role in T1D pathogenesis.
