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Optimization of Extraction Method of Anthocyanins from Red Cabbage
Auryclennedy Calou de Araújo1, Josivanda Palmeira Gomes1, Francilânia Batista da Silva1
1Center for Technology and Natural Resources, Academic Unit of Agricultural Engineering, Federal University of Campina Grande, Campina Grande 58429-900, Brazil.
Molecules (Basel, Switzerland)
|April 28, 2023
Summary
Red cabbage is a rich source of anthocyanins for natural dyes. Optimal extraction uses 25% alcohol at pH 4.0 and 25°C, yielding significantly higher pigment concentrations.
Area of Science:
- Food Science
- Natural Pigments
- Phytochemistry
Background:
- Red cabbage (Brassica oleracea var. capitata f. rubra) is a valuable source of anthocyanins, widely used in food production for natural colorants.
- Anthocyanins are plant pigments responsible for red, purple, and blue hues, with significant antioxidant properties.
Purpose of the Study:
- To optimize the extraction of anthocyanins from red cabbage for natural dye production.
- To investigate the impact of different solvents, pre-treatments, pH, and temperatures on anthocyanin yield and extract color.
Main Methods:
- Red cabbage was extracted using distilled water, 25% ethyl alcohol, and 70% ethyl alcohol.
- Extraction conditions included two pre-treatment groups (dried vs. raw), two pH levels (4.0 and 6.0), and two temperatures (25°C and 75°C), resulting in 24 formulations.
- Colorimetric parameters and anthocyanin content were analyzed for each extract.
Main Results:
- The optimal extraction conditions were identified as using 25% ethyl alcohol as the solvent, a pH of 4.0, and a processing temperature of 25°C.
- This specific method yielded a reddish extract with an average anthocyanin content of 191.37 mg/100 g.
- This represents a 74% increase in anthocyanin yield compared to other tested conditions and solvents.
Conclusions:
- The study successfully identified optimal parameters for maximizing anthocyanin extraction from red cabbage.
- The findings provide a basis for efficient industrial production of natural red dyes from red cabbage, enhancing natural colorant applications.

